Diagnostic value of serum protein electrophoresis and free light chains in the multiple myeloma's diagnosis
Keywords:
Multiple myeloma and diagnosis, blood protein electrophoresis, cellulose acetate electrophoresis, serum free light chains, diagnostic accuracyAbstract
Background:Protein electrophoresis and serum free light chains are techniques used in the diagnosis of multiple myeloma. However, the diagnostic utility of both tests may vary according to the method used and the actual conditions of the environment where they are performed.
Objective:To determine the diagnostic value of protein electrophoresis and serum free light chains in multiple myeloma.
Methodology:A retrospective study of serum protein electrophoresis parameters and serum free light chains was conducted in 43 patients diagnosed with multiple myeloma by bone marrow evaluation. Protein electrophoresis was completed by the conventional method of protein separation on cellulose acetate paper and for free light chains an immunoturbidimetric assay was applied in which a chemical analyzer (Cobas 311) was used. Seven parameters were calculated to evaluate diagnostic accuracy.
Results:All parameters assessing diagnostic accuracy were within confidence intervals in both tests.
Conclusions:Protein electrophoresis and serum free light chains are very useful assays in the diagnosis of multiple myeloma and should be used in conjunction for the highest possible approval of cases.
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